Isothermal strand displacement nucleic acid amplification

US6214587A · kind A · utility

Assignee

• GEN-PROBE INCORPORATED · US

Inventors

• Nanibhushan Dattagupta · San Diego, US
• Paul Douglas Stull · San Diego, US
• Marc Spingola · Albuquerque, US
• Daniel L. Kacian · San Diego, US

Key dates

• Filing date: Feb 17, 2000
• Grant date: Apr 10, 2001
• Expiry date: Feb 17, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6844
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5' exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.