Microcarrier beads having a styrene copolymer core and a covalently linked tri-methylamine exterior
US6214618A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 1999 |
| Grant date | Apr 10, 2001 |
| Priority date | — |
| Expiry date | Apr 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There is provided a method of making microcarrier beads having the steps of forming a bead made of a lightly crosslinked styrene copolymer core and also having functional groups on the surface of the bead and washing the microcarrier beads with basic and acidic solutions to make the beads compatible for cell culture. Also provided is a microcarrier bead made of a styrene copolymer core with a tri-methylamine exterior which has been washed in basic and acidic solutions to make the beads compatible for cell culture. The method of using microcarrier beads for increased growth of anchorage dependent cells having the steps of washing the microcarrier bead with basic and acidic solutions and mixing the microcarrier bead with an anchorage dependent cell containing culture medium is also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.