Composition and method for differentiation of basophils and eosinophils in blood
US6214625A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 28, 1999 |
| Grant date | Apr 10, 2001 |
| Priority date | — |
| Expiry date | Apr 28, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to lytic reagents and methods of using the lytic reagents for automatically determining leukocyte subpopulations in blood. More specifically, the new lytic reagents selectively lyse red blood cells and certain leukocyte subpopulations, which enables the differentiation of at least one subpopulation of leukocytes. The lytic reagents contain an ethoxylated long chain amine, a quaternary ammonium salt and an acid. When used in combination with a second lytic reagent system, one is able to obtain at least a five part differential of leukocytes by impedance and light scatter measurements, by impedance and radio frequency measurements, or by radio frequency and light scatter measurements.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.