Enzymatic method for removal of cryoprotectants from cryopreserved animal cells
US6218101A · kind A · utility
0Cited by
7References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 4, 1999 |
| Grant date | Apr 17, 2001 |
| Priority date | — |
| Expiry date | May 4, 2019 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01N1/12
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
A method to remove cryoprotectants from cryopreserved biological cells and tissues is described. Enzymes are used to convert compounds used as cryoprotectants for cells during cryopreservation to membrane impermeable products. This process effectively removes the cryoprotectant chemicals from the cells prior to their use in transfusion, transplantation, insemination or other applications, without causing osmotic damage due to cell swelling. Methods are described for subsequent removal of the enzymes and enzyme conversion products from the cell and tissue preparations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.