Construction of normalized cDNA libraries from eucaryotic cells

US6218123A · kind A · utility

Assignee

• Lexicon Genetics, Inc. · US

Inventors

• Michael Nehls · Stockdorf, DE
• Brian Zambrowicz · Diemelstraße, US
• Glenn Friedrich · Diemelstraße, US
• H. Earl Ruley · Nashville, US
• Arthur Sands · Diemelstraße, US
• Sigrid Wattler · Diemelstraße, US

Key dates

• Filing date: Aug 10, 1999
• Grant date: Apr 17, 2001
• Expiry date: Aug 10, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1096
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A new technology is described that allows for the rapid and efficient construction of complex cDNA libraries from cultured eukaryotic cells. The technology exploits eukaryotic biology by using transgenic constructs that have been nonspecifically inserted into the genome to facilitate the expression of nuclear genes as fusion transcripts. The invention further allows one to specifically subclone the corresponding fusion transcripts into highly complex cDNA libraries. The libraries are easily characterized by molecular analysis techniques such as hybridization, and individual clones can be directly sequenced to generate a sequence database of the cellular portion of the fusion transcripts.