Enhancer for eukaryotic expression systems

US6218140A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Bernhard Fleckenstein · Wiesenthau, DE
• Walter E. Schaffner · Shavertown, US
• Frank Weber · Essen, DE
• Karoline Dorsch-Hasler · Zürich, CH
• Gerhard Jahn · Neunkirchen, DE
• Michael Boshart · Heidelberg, DE

Key dates

• Filing date: Nov 9, 1994
• Grant date: Apr 17, 2001
• Expiry date: Nov 9, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An enhancer has been located in the upstream region of the major immediate early gene of human cytomegalovirus and has been isolated, which enhancer is more active than that from SV40 and has a wide host cell spectrum. Hence, it is suitable for eukaryotic expression systems wherein it can be incorporated upstream or downstream of the structural gene or of the regulation region.