Tissue specific hypoxia regulated constructs

US6218179A · kind A · utility

Assignee

• SRI International · US

Inventors

• Keith A. Webster · Palo Alto, US
• Nanette Bishopric · Palo Alto, US
• Brian Murphy · Palo Alto, US
• Keith Laderoute · Menlo Park, US
• Christopher J. Green · Halifax, GB

Key dates

• Filing date: Jun 23, 1997
• Grant date: Apr 17, 2001
• Expiry date: Jun 23, 2017

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions relating to chimeric genes containing (i) a tissue-specific promoter and (ii) a hypoxia response enhancer element, both of which are operably linked to a selected gene, such as a reporter gene, therapeutic gene (e.g., bcl-2, NOS, catalase and SOD), or deleterious gene are disclosed. Expression of the selected gene is enhanced in the target tissue under hypoxia conditions, such as conditions encountered during episodes of ischemia and reperfusion. The methods and compositions may be used as therapeutics and/or diagnostics.