Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US6218187A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 11, 1999 |
| Grant date | Apr 17, 2001 |
| Priority date | — |
| Expiry date | Mar 11, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13062
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.