Method of isolating RNA
US6218531A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 20, 1999 |
| Grant date | Apr 17, 2001 |
| Priority date | — |
| Expiry date | Dec 20, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for isolating RNA from a biological material comprising RNA and contaminants, wherein: the biological material is disrupted in the presence of a chaotropic agent, the resulting lysate is diluted to precipitate out contaminants, and the precipitate is removed from the lysate. RNA is preferably isolated from the resulting cleared lysate, using a silica matrix to bind and then release RNA bound thereto under particular conditions. The present invention also provides a method for isolating RNA from a solution comprising RNA and DNA, wherein: the RNA and DNA are bound to a silica matrix in the presence of at least one binding enhancer, the DNA is digested with DNase, and the RNA fluted therefrom.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.