Enzymatic-electrochemical one-shot affinity sensor for the quantitative determination of analytes for aqueous media and affinity assay

US6221238A · kind A · utility

Assignees

• UFZ-Umweltforschungs Zentrum Leipzig-Halle GmbH · DE
• SensLab Gesellschaft zur Entwicklung und Herstellung bioelektrochemischer Sensoren mbH · DE

Inventors

• Bernd Gründig · Leipzig, DE
• Ilka Renneberg · Berlin, DE
• Beate Strehlitz · Leipzig, DE
• Holm Kopinke · Leipzig, DE

Key dates

• Filing date: Feb 16, 1999
• Grant date: Apr 24, 2001
• Expiry date: Feb 16, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to an enzymatic-electrochemical affinity sensor and a one-step affinity assay for the quantitative determination of analytes in aqueous media. More specifically, the invention relates to an enzymatic-electrochemical signal amplification system for a highly sensitive indication of affinity reactions and is particularly suitable in the form of a one-step affinity sensor for in situ analytics. The invention is also directed to the use of phenol oxidase as a marker enzyme for the affine binding partners in an electrochemical affinity sensor or assay, and to the use of an enzyme hydrolyzing phenolic compounds as marker enzyme for the affine binding partners, in combination with a phenol oxidase as catalyst for the amplifying reaction in an electrochemical affinity assay.