Transgenic non-human mammal expressing the DNA sequence encoding kappa casein mammary gland and milk

US6222094A · kind A · utility

Assignee

• Symbicom Aktiebolag · SE

Inventors

• Lennart Hansson · Tomelilla, SE
• Mats Stromqvist · Wasserburg am Inn, DE
• Sven Bergstrom · Honeytree Loop East, SE
• Olle Hernell · Umeå, SE
• Jan Törnell · Mölndal, SE

Key dates

• Filing date: Dec 6, 1994
• Grant date: Apr 24, 2001
• Expiry date: Dec 6, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to an expression system comprising a DNA sequence encoding a polypeptide which ha a biological activity of human .kappa.-casein, the system comprising a 5'-flanking sequence capable of mediating expression of said DNA sequence. In preferred embodiments the 5'-flanking sequence is from a milk protein gene of a mammal such as a casein gene or whey acidic protein (WAP) gene and the DNA sequence contains at least one intron sequence. The invention further relates to DNA sequences, replicable expression vectors and cells harboring said vectors, recombinant polypeptide e.g. in glycosylated form, and milk, infant formula or nutrient supplement comprising recombinant polypeptide. The invention also relates to a method for producing a transgenic non-human mammal comprising injecting an expression system as defined above and optionally a further DNA encoding .beta.-casein or an analog, variant or subsequence thereof into a fertilized egg or a cell of an embryo of a mammal so as to incorporate the expression system into the germline of the mammal and developing the resulting injected fertilized egg or embryo into an adult female mammal. In one embodiment, the end…