Patent · US Expired

Systemic gene therapy by intestinal cell transformation

US6225290A · kind A · utility

45Cited by
6References
14Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 19, 1996
Grant dateMay 1, 2001
Priority date
Expiry dateSep 19, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K14/61
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Intestinal epithelial cells of a mammalian subject are genetically altered to operatively incorporate a gene which expresses a protein which has a desired therapeutic effect. Intestinal cell transformation is accomplished by administration of a formulation composed primarily of naked DNA, and is preferably administered orally. Oral or other intragastrointestinal routes of administration provide a simple method of administration, while the use of naked nucleic acid avoids the complications associated with use of viral vectors to accomplish gene therapy. The expressed protein is secreted directly into the gastrointestinal tract and/or blood stream to obtain therapeutic blood levels of the protein thereby treating the patient in need of the protein. The transformed intestinal epithelial cells provide short or long term therapeutic cures for diseases associated with a deficiency in a particular protein or which are amenable to treatment by overexpression of a protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.