Non-radioactive hybridization assay and kit
US6228578A · kind A · utility
49Cited by
6References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 18, 1994 |
| Grant date | May 8, 2001 |
| Priority date | — |
| Expiry date | Jan 18, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/301
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A non-radioactive hybridization assay and kit for the detection of genetic defects, microbial infections or viral infections, such as human papillomavirus. A test sample is treated with a base and incubated with nucleic acid probes, diluted in a neutralizing buffer, specific for target nucleic acids. The hybrids are captured onto a solid phase coated with an anti-hybrid antibody, unhybridized probe is eliminated, and the bound hybrid detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.