Nucleic acid detection method using nucleotide probes enabling both specific capture and detection
US6228580A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 30, 1998 |
| Grant date | May 8, 2001 |
| Priority date | — |
| Expiry date | Jan 30, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nucleic acid detection assay is disclosed which assay comprises hybridizing a large nucleic acid probe (i.e. preferably more than 100 bp long) with a RNA or DNA target nucleic acid present in a biological sample. The probe in this method is to comprise both a detection element and a capture element and is to to be degradable by a suitable enzyme when it is single stranded. Following hybridization the sample is treated with a enzyme capable of degrading unhybridized nucleic acids. Then the hybrids are captured by contacting them with a solid support comprising an element capable of binding with the capture element of the probe. Finally the detection element of the probe is used to detect the probe, thereby indicating that the target nucleic acid is present in the biological sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.