Methods for enhanced production of tissue plasminogen activator in cell culture using alkanoic acids or salts thereof
US6228618A · kind A · utility
1Cited by
1References
8Claims
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Key dates
| Filing date | Oct 22, 1997 |
| Grant date | May 8, 2001 |
| Priority date | — |
| Expiry date | Oct 22, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for enhancing the production of tissue plasminogen activator (tPA) in cell culture are disclosed. The methods involve culturing tPA-producing cells in growth media supplemented with an alkanoic acid or salt thereof at a concentration which enhances tPA production. The most preferred methods utilize butyric acid or sodium butyrate at a concentration of between 0.5 mM and 2.5 mM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.