Leucine aminopeptidases produced recombinantly from Aspergillus soyae

US6228632A · kind A · utility

Assignee

• Roehm GmbH · DE

Inventors

• Erwin Schuster · Bechhofen, DE
• Bruno Sproessler · Roßdorf, DE
• Kornelia Titze · Heilbad Heiligenstadt, DE
• Michael J. Gottschalk · Ober-Ramstadt, DE
• Nguyen Quoc Khanh · Reichelsheim, DE
• Sabine Wolf · Otzberg, DE
• Hermann Plainer · Reinheim, DE

Key dates

• Filing date: Apr 20, 1998
• Grant date: May 8, 2001
• Expiry date: Apr 20, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a recombinant deoxyribonucleic acid (DNA) which can be isolated from Aspergillus soyae, characterised in that it codes for a leucine aminopeptidase (LAP) and comprises a nucleotide sequence corresponding to the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP or to a nucleotide sequence derived therefrom which hybridises under stringent conditions with the nucleotide sequence given in SEQ ID NO: 1 for the mature LAP. The invention further relates to vectors and transformed host organisms, and also relates to methods of producing LAP. Enzyme products for the production of protein hydrolysates, as well as protein hydrolysates which are produced correspondingly, also form part of the invention.