Patent · US Expired

High throughput assay system

US6232066A · kind A · utility

167Cited by
23References
41Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 2, 1998
Grant dateMay 15, 2001
Priority date
Expiry dateJul 2, 2018

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC40B40/06
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

The present invention relates to compositions, apparatus and methods useful for concurrently performing multiple, high throughput, biological or chemical assays, using repeated arrays of probes. A combination of the invention comprises a surface, which comprises a plurality of test regions, at least two of which, and in a preferred embodiment, at least twenty of which, are substantially identical, wherein each of the test regions comprises an array of generic anchor molecules. The anchors are associated with bifunctional linker molecules, each containing a portion which is specific for at least one of the anchors and a portion which is a probe specific for a target of interest. The resulting array of probes is used to analyze the presence or test the activity of one or more target molecules which specifically interact with the probes. In one embodiment of the invention, the test regions (which can be wells) are further subdivided into smaller subregions (indentations, or dimples).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.