Heterogeneous assay for pyrophosphate detection
US6232075A · kind A · utility
107Cited by
22References
30Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 13, 1999 |
| Grant date | May 15, 2001 |
| Priority date | — |
| Expiry date | Dec 13, 2019 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Nucleotide triphosphate probes containing a fluorophore attached to the .beta.-phosphate and a quencher moiety sufficiently proximal to the fluorophore moiety for use in pyrophosphate detection assays are disclosed. These probes exhibit distinguishable fluorescence characteristics when the fluorophore is attached to the nucleotide through the .gamma.-phosphate and when it is unattached to the nucleotide. The present invention also provides kits and integrated systems for practicing the assays described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.