DNA encoding human .kappa. casein and process for obtaining the protein

US6232094A · kind A · utility

Assignee

• Symbicom Aktiebolag · SE

Inventors

• Lennart Hansson · Tomelilla, SE
• Mats Stromqvist · Wasserburg am Inn, DE
• Sven Bergstrom · Honeytree Loop East, SE
• Olle Hernell · Umeå, SE
• Jan Törnell · Mölndal, SE

Key dates

• Filing date: Jun 5, 1995
• Grant date: May 15, 2001
• Expiry date: Jun 5, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/44
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to an expression system comprising a DNA sequence encoding a polypeptide which has a biological activity of human .kappa.-casein, the system comprising a 5'-flanking sequence capable of mediating expression of said DNA sequence. In preferred embodiments the 5'-flanking sequence is from a milk protein gene of a mammal such as a casein gene or whey acidic protein (WAP) gene and the DNA sequence contains at least one intron sequence selected from the intron sequences presented in SEQ ID NO:30. The invention also relates to DNA sequences, replicable expression vectors and cells harboring said vectors, recombinant polypeptide e.g. in glycosylated form, and milk, infant formula or nutrient supplement comprising recombinant polypeptide. The invention further relates to a transgenic non-human mammal such as a mouse, rat, rabbit, goat, sheep, pig, lama, camel or bovine species whose germ cells and somatic cells contain a DNA sequence as defined above as a result of chromosomal incorporation into the non-human mammalian genome, or into the genome of an ancestor of said non-human mammal.