Patent · US Expired

Conditional replication and expression system

US6232105A · kind A · utility

5Cited by
1References
26Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 16, 1999
Grant dateMay 15, 2001
Priority date
Expiry dateDec 16, 2019

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2750/14152
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The present invention relates to the utilization of conditionally replicating recombinant nucleic acid molecules rescued from the integrated state for the expression of foreign proteins. The usefulness of the system is illustrated with a conditionally replicating recombinant nucleic acid molecule encoding the adeno-associated virus (AAV) capsid proteins. The present invention also relates to methods employing and conditionally replicating recombinant nucleic acid molecules for the packaging of recombinant AAV nucleic acid molecule into AAV capsids. The present invention also relates to packaging cell lines for recombinant AAV, expressing both the AAV rep and cap-genes.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.