Reduction of nonspecific hybridization by using novel base-pairing schemes
US6232462A · kind A · utility
83Cited by
4References
6Claims
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Assignee
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Key dates
| Filing date | Jul 14, 1998 |
| Grant date | May 15, 2001 |
| Priority date | — |
| Expiry date | Jul 14, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/336
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.