Malathion carboxylesterase

US6235515A · kind A · utility

Assignee

• COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION · AU

Inventors

• Robyn Russell · Tuggeranong, AU
• Richard David Newcomb · National Forest Development Road 091, AU
• Peter Malcolm Campbell · Belconnen, AU
• Geoffrey Charles de Quetteville Robin · North Canberra, AU
• Charles Claudianos · Tuggeranong, AU
• Kerrie-Ann Smyth · Syracuse, US
• Thomas Mark Boyce · Belconnen, AU
• John Oakeshott · Tuggeranong, AU
• Jeremy Colin Brownlie · Queanbeyan, AU

Key dates

• Filing date: May 20, 1998
• Grant date: May 22, 2001
• Expiry date: May 20, 2018

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A62D2101/26
• WIPO field: Machine tools
• WIPO sector: Mechanical engineering

Abstract

The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyloxon organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc.alpha.E.sub.7, in which the protein encoded by the DNA molecule differs from E.sub.3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.