Phage bonded to a nuclear location signal
US6235521A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 10, 1999 |
| Grant date | May 22, 2001 |
| Priority date | — |
| Expiry date | Sep 10, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10343
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A .lambda. phage with a nuclear localization signal has been obtained by constructing a vector capable of expressing a fused protein between a gpD protein constituting the head of a .lambda. phage and a nuclear localization signal sequence, transforming Escherichia coli with this vector, and propagating a mutant .lambda. phage which cannot express the gpD protein in E. coli in this transformant. It has been confirmed that the resulting .lambda. phage is capable of packaging .lambda. phage DNAs of 80% and 100% genome sizes. After further confirming that the nuclear localization signal exposed on the outside of the head of this phage, this phage has been microinjected into cells to analyze its nuclear localization activity. Thus, it has been clarified that this phage has a nuclear localization activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.