Patent · US Expired

Analysis of quiescent anticoagulated whole blood samples

US6235536A · kind A · utility

145Cited by
7References
21Claims
0Family size

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Key dates

Filing dateFeb 12, 1999
Grant dateMay 22, 2001
Priority date
Expiry dateFeb 12, 2019

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25375
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Formed constituents of a quiescent anticoagulated whole blood sample are optically or visually analyzed in a sample chamber which has a varying through plane thickness due to convergent opposing sample chamber walls. At least one of the convergent walls of the chamber is transparent so that the blood sample constituents can be observed. The chamber's varying thickness produces a first lesser thickness region in the chamber wherein a quiescent monolayer of red blood cells in the sample will reside after the sample is introduced into and fills the chamber. Larger formed constituents such as white blood cells in the sample are unable to enter the aforesaid lesser thickness region of the chamber. The red cells which reside in the greater thickness regions will agglomerate to form rouleaux and lacunea. The exact thickness of the chamber at any particular location in the chamber can be predetermined, or can be determined in situ as the sample is being analyzed. By admixing certain dyes with the blood sample, various characteristics and other information can be derived from the various formed constituents in the sample by means of a scanning instrument which is able to measure various col…

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.