CICM cells and non-human mammalian embryos prepared by nuclear transfer of a proliferating differentiated cell or its nucleus
US6235970A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 22, 1997 |
| Grant date | May 22, 2001 |
| Priority date | — |
| Expiry date | Sep 22, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2517/04
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
An improved method of nuclear transfer involving the transplantation of donor differentiated cell nuclei into enucleated oocytes of the same species as the donor cell is provided. The resultant nuclear transfer units are useful for multiplication of genotypes and transgenic genotypes by the production of fetuses and offspring, and for production of isogenic CICM cells, including human isogenic embryonic or stem cells. Production of genetically engineered or transgenic mammalian embryos, fetuses and offspring is facilitated by the present method since the differentiated cell source of the donor nuclei can be genetically modified and clonally propagated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.