Acetic acid as a signal enhancing contrast agent in fluorescence spectroscopy
US6241662A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 20, 1998 |
| Grant date | Jun 5, 2001 |
| Priority date | — |
| Expiry date | Oct 20, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1488
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for using acetic acid as a signal enhancing contrast agent during fluorescence spectroscopy of normal and neoplastic tissue, particularly epithelium. In one aspect, the invention includes a method of detecting tissue abnormality in a diagnostic tissue sample in a patient, comprising the steps of obtaining a first fluorescence intensity spectrum from the diagnostic tissue sample; thereafter, applying acetic acid to the diagnostic tissue sample in sufficient concentration to alter the response of such diagnostic tissue sample to electromagnetic radiation for at least an effective period of time; during the effective period of time, obtaining a second fluorescence intensity spectrum from the diagnostic tissue sample; determining a parameter indicative of a change between the first and second fluorescence emission intensity spectra; and analyzing the determined parameter to determine a probability that the diagnostic tissue sample is normal or abnormal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.