Genomic polyphenol oxidase gene fragments of plants
US6242221A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 4, 1998 |
| Grant date | Jun 5, 2001 |
| Priority date | — |
| Expiry date | Aug 4, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/0059
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the isolation of polyphenol oxidase (PPO) gene fragments from plants. In a first aspect of the present invention there is provided a method for preparing PPO gene fragments, which method includes providing a sample of plant tissue, a first primer in sense orientation having a sequence corresponding to a conserved region of a PPO gene, a second primer in antisense orientation having a sequence corresponding to a conserved region of a PPO gene; isolating genomic DNA from the plant tissue; and amplifying the genomic DNA using the first and second primers. Surprisingly, the applicant has found that PPO genes in plants lack introns and, therefore, fragments of PPO genes may be amplified directly from genomic DNA of a range of plants. The lack of introns means that the size of the fragments can be predicted and bands of the appropriate size can be selected for cloning.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.