Method for purification of recombinant proteins
US6242581A · kind A · utility
10Cited by
1References
5Claims
0Family size
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Key dates
| Filing date | Aug 18, 1997 |
| Grant date | Jun 5, 2001 |
| Priority date | — |
| Expiry date | Aug 18, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Purification of poly-amino acid-tagged recombinant proteins has been improved by the use of a carboxymethylated aspartate ligand complexed with a third-block transition metal having an oxidation state of 2.sup.+ and a coordination number of 6. A method for synthesizing the metal ion-CM-Asp complex is also described. Further, the metal ion-CM-Asp complex can be used for screening protein function.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.