Purification of peptides and oligonucleotides by sample displacement chromatography process and apparatus
US6245238A · kind A · utility
Inventor
Key dates
| Filing date | Oct 15, 1999 |
| Grant date | Jun 12, 2001 |
| Priority date | — |
| Expiry date | Oct 15, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/466
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Sample displacement chromatography is performed at low operating pressures (less than 30 bar) and/or at high sample loading (in excess of 500 mg/cm.sup.2 column area), with product recovery being effected in a non-gradient manner. The low operating pressures permit the use of simple and inexpensive apparatus. Non-gradient product recovery allows the desired product to be allowed in solutions with advantageously high concentration. Materials which may be purified include pharmaceuticals, pharmaceutical excipients, fine chemicals, biochemicals, X-ray contrast agents, chelating agents, peptides, proteins, oligonucleotides and vaccines. Apparatus embodiments include sample displacement chromatography apparatus comprising one or more ion exchange columns in direct combination with one or more desalting columns, and multicolumn chromatography apparatus permitting switching between a series separation mode and a parallel extraction mode.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.