Polynucleotide arrays and methods of making and using the same
US6245518A · kind A · utility
35Cited by
7References
33Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 6, 1999 |
| Grant date | Jun 12, 2001 |
| Priority date | — |
| Expiry date | Dec 6, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B60/14
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to methods of using labeled tracers to generate spatially addressable arrays of immobilized molecules, particularly polynucleotides, that can be normalized for differences in immobilization efficiencies at different addresses in the array. It also relates to the arrays generated by the method and to use of these arrays to enhance discrimination in array-based assays, particularly the discrimination between perfectly matched hybrids and hybrids containing a single mismatch in nucleic acid hybridization assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.