Method for cloning and producing the SwaI restriction endonuclease
US6245545A · kind A · utility
7Cited by
5References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 27, 1999 |
| Grant date | Jun 12, 2001 |
| Priority date | — |
| Expiry date | Apr 27, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.