Water soluble tri-substituted 1,2-dioxetane compounds having increased storage stability, synthetic processes and intermediates
US6245928A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 17, 2000 |
| Grant date | Jun 12, 2001 |
| Priority date | — |
| Expiry date | Feb 17, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07C2603/74
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Stable, enzymatically triggered chemiluminescent 1,2-dioxetanes with improved water solubility and storage stability are provided as well as synthetic processes and intermediates used in their preparation. Dioxetanes further substituted with two or more water-solubilizing groups disposed on the dioxetane structure and an additional fluorine atom or lower alkyl group provide superior performance by eliminating the problem of reagent carryover when used in assays performed on capsule chemistry analytical systems. These dioxetanes display substantially improved stability on storage. Compositions comprising these dioxetanes, a non-polymeric cationic surfactant enhancer and optionally a fluorescer, for providing enhanced chemiluminescence are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.