Labeled primer for use in and detection of target nucleic acids
US6248526A · kind A · utility
72Cited by
3References
22Claims
0Family size
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Inventor
Key dates
| Filing date | Dec 14, 1998 |
| Grant date | Jun 19, 2001 |
| Priority date | — |
| Expiry date | Dec 14, 2018 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A labeled primer for use in detection of nucleic acid is described, which primer is labeled at the two ends of the oligonucleotide strand with a reporter dye molecule and a quencher molecule, and in which labeled primer, at least one base at the 3' end is deliberately not complementary to the nucleic acid sequence to be amplified. A process for detecting a nucleic acid sequence using a labeled primer is also described. The process can also be used in amplification processes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.