Template-specific termination in a polymerase chain reaction
US6248567A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 2000 |
| Grant date | Jun 19, 2001 |
| Priority date | — |
| Expiry date | Apr 24, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein is a method for selectively inhibiting the amplification of a specific DNA template during a polymerase chain reaction (PCR). In particular, the method is useful when the sequences of the desired and undesired DNA templates are similar. A set of universal primers binds to both the desired and undesired DNA templates during a PCR, resulting in the amplification of their DNA sequences. The method targets the undesired DNA template with three sets of oligonucleotide primers, one set of which is terminally modified to both prevent primer extension and increase the primer-template binding affinity. The result of these terminal modifications is the specific inhibition of the PCR amplification of the undesired DNA template, allowing the preferential amplification of the desired DNA templates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.