Infectious clone for human parainfluenza virus type 3
US6248578A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 6, 1998 |
| Grant date | Jun 19, 2001 |
| Priority date | — |
| Expiry date | May 6, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/18652
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A system for generating recombinant, human parainfluenza virus, particularly infectious, recombinant, human parainfluenza virus type 3 (HPIV-3) is provided. In one embodiment, the system comprises a clone comprising a nucleotide sequence that encodes a full-length, positive sense, anti-genome of HPIV, and at least one support clone comprising a nucleotide sequence that encodes the HPIV P protein and the HPIV L protein. In another embodiment, the system further comprises a support clone which comprises a nucleotide sequence that encodes the HPIV NP protein. The present invention also provides a MAH clone which comprises a nucleotide sequence encoding the full-length, positive sense, anti-genome of HPIV-3. The clone also comprises an RNA polymerase promoter operatively linked to the HPIV-3 antigenome-encoding sequence. In a preferred embodiment, the clone further comprises a nucleotide sequence which encodes a ribozyme immediately downstream from the sequence encoding the HPIV-3 anti-genome. The present invention also relates to a method of preparing recombinant HPIV-3 virus having site-specific mutations in the HPIV-3 genome. The method comprises preparing a clone comprising a modif…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.