Purified chitinases and use thereof
US6251390A · kind A · utility
20Cited by
3References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 27, 1992 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Jul 27, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01052
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Two chitinases from Trichoderma harzianum P1 (ATCC 74058) show chitin-containing-fungus-inhibiting activity. One is an endochitinase and the other is a chitobiase. Both have molecular weights of 40 kDa and isoelectric points of 3.9. A .lambda.gtll recombinant encoding for the endochitinase has been prepared (ATCC 55338) and the gene encoding for the endochitinase has been removed from the DNA of the recombinant by the restriction enzyme, Not I. Endochitinases and chitobiases including the two purified from Trichoderma harzianum strain P1 demonstrate synergy with each other in anti-fungal effect.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.