Assay and reagents for identifying anti-proliferative agents
US6251585A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 1995 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Apr 24, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/16
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention makes available assays and reagents for identifying anti-proliferative agents, such as mitotic and meiotic inhibitors. The present assay provides a simple and rapid screening test which relies on scoring for positive cellular proliferation as indicative of anti-mitotic or anti-meiotic activity, and comprises contacting a candidate agent with a cell which has an impaired cell-cycle checkpoint and measuring the level of proliferation in the presence and absence of the agent. The checkpoint impairment is such that it either causes premature progression of the cell through at least a portion of a cell-cycle or inhibition of normal progression of the cell through at least a portion of a cell-cycle, but can be off-set by the action of an agent which inhibits at least one regulatory protein of the cell-cycle in a manner which counter-balances the effect of the impairment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.