Process for determining a resistance to activated protein C
US6251619A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 5, 1999 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Feb 5, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/4716
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for determining a resistance to activated protein C of a test specimen of human plasma following the steps of: (1) mixing together (a) the test specimen of human plasma, (b) a reactant deficient in factor V which supplies at least most of the coagulation factors other than factor V, and (c) the venom of Crotalus viridis helleri which specifically activates factor X to Xa, and incubating the mixture of (a), (b) and (c) for at least one minute at a temperature of between 10 and 45.degree. C.; (2) introducing into the incubated mixture(i) Ca.sup.2+ or (ii) Ca.sup.2+ +exogenic activated protein C; and (3) determining the coagulation time (i) in the absence of activated protein C and (ii) in the presence of activated protein C. Steps (1) to (3) are repeated, but replacing, in step (1), the test specimen with a normal plasma as control and correlating resistance to activated protein C by comparing the determinations made in steps for the test specimen and for the normal plasma. The initiation of coagulation is caused by activating factor X to Xa using the venom of Crotalus viridis helleri in the presence of (i) Ca.sup.2+ or (ii) Ca.sup.2+ + exogenic activated protein C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.