Recombinant preparation of calcitonin fragments and use thereof in the preparation of calcitonin and related analogs

US6251635A · kind A · utility

Assignee

• Bionebraska, Inc. · US

Inventors

• Fred W. Wagner · Walton, US
• Jay Stout · Lincoln, US
• Dennis Henriksen · Lincoln, US
• Bruce E. Partridge · Lincoln, US
• Bart Holmquist · Lincoln, US
• Julie A. Frank · Lincoln, US

Key dates

• Filing date: Aug 25, 1998
• Grant date: Jun 26, 2001
• Expiry date: Aug 25, 2018

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.