Use of extremely thermophilic DNA-polymerases
US6251637A · kind A · utility
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2References
8Claims
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Key dates
| Filing date | Apr 7, 1999 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Apr 7, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cycle sequencing of DNA with marker-labeled dideoxynucleotides is performed using extremely thermophilic DNA polymerases in a one-lane technique in which dideoxynucleotides labeled with a dye as a marker are used to obtain sequence ladders of more 500 bases. Chain elongation is performed at a temperature of 65 to 75.degree. C. Surprisingly, it was found that chain elongation temperatures of substantially more than 60.degree. C. could be used in cycle sequencing of DNA with marker labeled dye dideoxynucleotides when using extremely thermophilic DNA polymerases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.