Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer
US6251639A · kind A · utility
502Cited by
42References
116Claims
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Inventor
Key dates
| Filing date | Sep 13, 2000 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Sep 13, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides novel isothermal, single primer linear nucleic acid amplification methods. Methods for amplifying complementary DNA using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.