Fluorescence filter cube for fluorescence detection and imaging
US6252664A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 15, 1999 |
| Grant date | Jun 26, 2001 |
| Priority date | — |
| Expiry date | Oct 15, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/59
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence cube comprising a housing; an exciter filter which allows passage of an incident light comprising a spectral range of from about 200 nanometers to about 400 nanometers; and either a beam splitter or a dichroic mirror which directs the incident light in a desired direction, and which transmits an emitted light in a desired direction. Also disclosed is a method of using the fluorescence cube for acquiring multicolor fluorescence images, the method comprising fluorescence labeling of a substrate with more than one species of water-soluble nanocrystals; and imaging the labeled substrate with a detection system comprising the fluorescence cube by exposing the labeled substrate to an incident light comprising an excitation spectral range, and detecting a transmitted light comprising an emission spectral range.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.