Nitric oxide-scavenging system for culturing oocytes, embryos, or other cells

US6255109A · kind A · utility

Assignee

• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US

Inventors

• William Hansel · Baton Rouge, US
• Jeong Mook Lim · Seoul, KR

Key dates

• Filing date: Jun 17, 1999
• Grant date: Jul 3, 2001
• Expiry date: Jun 17, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2517/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Nitric oxide adversely affects survival and development of cells such as oocytes and embryos in vitro, particularly in a co-culture system. The addition of a nitric oxide inhibitor such as hemoglobin to such systems eliminates this toxic effect, and promotes mammalian oocytes, embryos, or other cells in vitro.