Process for the one-stage resalting and purification of oligopeptides

US6258933A · kind A · utility

Assignee

• Degussa-Huels AG · DE

Inventors

• Kurt A. Gunther · Woodstock, US
• Franz Kunz · Fairview Avenue, DE
• Karlheinz Drauz · Alzenau, DE
• Thomas Muller · Bad Homburg vor der Höhe, DE

Key dates

• Filing date: Mar 26, 1999
• Grant date: Jul 10, 2001
• Expiry date: Mar 26, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/36
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a process for the one-stage resalting and purification of oligopeptides. Oligopeptides are often not formed directly as acetates when synthesised. Acetate salts of oligopeptides are however desirable as bulk-active material for medical and formulation reasons. Processes known from the prior art have hitherto involved two separate steps or pyridine-containing solvents. The resalting and purification can be combined in one step and the use of pyridine as solvent can be avoided, if the oligopeptide in the form of its chloride salt is purified with an acetate-containing solvent by liquid chromatography methods.