Method for the purification and isolation of blood clotting proteins using conformation specific antibodies
US6258938A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 1992 |
| Grant date | Jul 10, 2001 |
| Priority date | — |
| Expiry date | Aug 18, 2012 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of isolating a protein from a mixture containing the protein, the method including providing an antibody immobilized on a solid support, which antibody is reactive with the protein complexed with a ligand and substantially unreactive with the protein not complexed with the ligand; contacting the mixture, in the presence of the ligand, with the immobilized antibody to bind the protein, complexed with the ligand, to the immobilized antibody to form an immune complex; and contacting the immune complex with a compound having a binding affinity for the ligand higher than the binding affinity of the protein for the ligand, to release the protein from the immobilized antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.