Method and apparatus for detection of multiple nucleic acid sequences and multiple antigens
US6261771A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 9, 1998 |
| Grant date | Jul 17, 2001 |
| Priority date | — |
| Expiry date | Nov 9, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/682
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and apparatus for detection of multiple target nucleic acids and/or antigens such as hormones, antibodies, or nerve agents in a sample, involves presenting the sample to a plurality of reporter binding sites wherein each reporter binding site comprises two partially hybridized molecules. A first of the two hybridized molecules is bound to the binding site and is complementary to a target nucleic acid or antigen, and it will therefore hybridize to the target nucleic acid or antigen and cause the release of the second hybridized molecule into the sample. The second hybridized molecule comprises a reporter nucleic acid sequence, which uniquely identifies the target nucleic acid or antigen. Subsequent PCR amplification of the unique reporter nucleic acid sequence using labeled primers results in multiple labeled copies of the unique nucleic acid sequence. The sample with the amplified and labeled copies of the unique nucleic acid sequence is then presented to a plurality of different collector binding sites where at least one of the sites comprises at least one collector molecule complimentary to the unique nucleic acid sequence. Unique nucleic acid sequences in the sample sel…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.