Method for marking solid, liquid or gaseous substances

US6261809A · kind A · utility

Assignee

• november Aktiengesellschaft Gesellschaft fur Molekulare Medizin · DE

Inventors

• Wolf Bertling · Erlangen, DE
• Hans Kosak · Bonn, DE

Key dates

• Filing date: Mar 3, 2000
• Grant date: Jul 17, 2001
• Expiry date: Mar 3, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/68
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for marking solid, liquid or gaseous substances, whereby the substance to be marked is provided with at least one synthetically produced nucleic acid sequence. Said nucleic acid sequence contains a first sequence section constructed with the 5' terminal end, a second sequence comprised of at least two bases and connected to said nucleic acid sequence, and a third sequence section constructed with the 3' terminal end and connected to the said nucleic acid sequence. In order to simplify the identification of the marking, the invention provides that a first primer group is used with a first primer section corresponding to the first sequence section and a second primer group is used with a third primer section corresponding to the third sequence section. Every primer group four comprises differing primer variants in at least one additional respective base which is provided on the end so that exactly one primer variant of the first primer group together with exactly one primer variant of the second primer group is complimentary to the nucleic acid sequence.