Method of introducing exogenous compounds into cells by electroporation and apparatus for same
US6261815A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 8, 1999 |
| Grant date | Jul 17, 2001 |
| Priority date | — |
| Expiry date | Jan 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M35/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An electroporation apparatus for introducing exogenous material into cells is described herein. The apparatus comprises first a base member configured for holding a cell support, the cell support having a top surface portion, with the top surface portion configured for carrying adherent cells. The apparatus further comprises an electrode carrier operably associated with the base member, the electrode carrier having a bottom surface portion, a first electrode connected to the electrode carrier, and a second electrode also connected to the electrode carrier. The electrode carrier has a channel formed therein, with the channel positioned between the first electrode and the second electrode, so that exogenous material may be introduced through the channel and into contact with the cells. Methods for introducing exogenous compounds into a cell and for visually detecting the location of binding events within a cell are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.