Process for modifying the stability of antibodies

US6262238A · kind A · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Boris Steipe · Gauting, DE
• Stefan Steinbacher · Lenggries, DE

Key dates

• Filing date: Sep 21, 1998
• Grant date: Jul 17, 2001
• Expiry date: Sep 21, 2018

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K39/39591
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Process for the production of a functional immunoglobulin or functional derivative or fragment thereof with an improved stability in a eukaryotic or prokaryotic organism by transformation with an expression vector which contains a recombinant gene which codes for the said immunoglobulin derivative or fragment which is characterized in that a modified gene is used which contains at least one codon which is substituted compared to the unmodified gene and codes for a more frequent amino acid. In this way it is also possible to produce active antibodies free of disulfide bridges. In a modification of the process it is possible to selectively destabilize antibodies.