RPS gene family, primers, probes, and detection methods

US6262248A · kind A · utility

Assignees

• MASSACHUSETTS GENERAL HOSPITAL · US
• The United States of America, as represented by the Secretary of Agriculture · US
• COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION · AU
• The Regents of the University of California · US

Inventors

• Frederick M. Ausubel · Newton, US
• Brian J. Staskawicz · Castro Valley, US
• Andrew F. Bent · Madison, US
• Douglas Dahlbeck · Castro Valley, US
• Fumiaki Katagiri · San Diego, US
• Barbara N. Kunkel · Chesterfield, US
• Michael N. Mindrinos · Somerville, US
• Guo-Liang Yu · Berkeley, US
• Barbara Baker · Richmond, US
• Jeffrey Ellis · Belconnen, AU
• John Salmeron · Hillsborough, US

Key dates

• Filing date: Apr 28, 1999
• Grant date: Jul 17, 2001
• Expiry date: Apr 28, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is substantially pure DNA encoding an Arabidopsis thaliana Rps2 polypeptide; substantially pure Rps2 polypeptide; and methods of using such DNA to express the Rps2 polypeptide in plant cells and whole plants to provide, in transgenic plants, disease resistance to pathogens. Also disclosed are conserved regions characteristic of the RPS family and primers and probes for the identification and isolation of additional RPS disease-resistance genes.